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SDS-PAGE is a method by which researchers can denature and separate proteins by size, allowing identification and even quantification of proteins of interest as well as downstream applications such as mass spectrometry to determine protein identities in a mixed sample. SDS is an ionic detergent used to unravel protein tertiary structure; addition of reducing agents like beta-mercaptoethanol also helps to eliminate disulfide bridges. The resultant denatured proteins are then subjected to an elect
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